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Literature summary extracted from

  • Zhang-Akiyama, Q.M.; Morinaga, H.; Kikuchi, M.; Yonekura, S.; Sugiyama, H.; Yamamoto, K.; Yonei, S.
    KsgA, a 16S rRNA adenine methyltransferase, has a novel DNA glycosylase/AP lyase activity to prevent mutations in Escherichia coli (2009), Nucleic Acids Res., 37, 2116-2125.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.182 expression of a pGEX-KsgA fusion construct in Escherichia coli KSR7 Escherichia coli
4.2.99.18 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.182 30400
-
 x * 30400, SDS-PAGE, calculated from sequence Escherichia coli
4.2.99.18 30000
-
 SDS-PAGE Escherichia coli
4.2.99.18 30400
-
 calculated from amino acid sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.182 Escherichia coli P06992
-
-
4.2.99.18 Escherichia coli
-
-
-
4.2.99.18 Escherichia coli KSR7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.182
-
 Escherichia coli
4.2.99.18 HiTrap column chromatography, HiTrap heparin column chromatography, HiTrap Blue column chromatography, glutathione-Sepharose 4B column chromatography, hydroxyapatite column chromatography, and Resource S column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.99.18 DNA KsgA has al DNA glycosylase/AP lyase activity for C mispaired with oxidized T Escherichia coli fragments of DNA
-
 ?
4.2.99.18 DNA KsgA has al DNA glycosylase/AP lyase activity for C mispaired with oxidized T Escherichia coli KSR7 fragments of DNA
-
 ?
4.2.99.18 additional information KsgA does not remove C opposite normal bases, 7,8-dihydro-8-oxoguanine and 2-hydroxyadenine, KsgA does not excise thymine glycol, 5-formyluracil, and 5-hydroxymethyluracil opposite cytosine from double-stranded oligonucleotides Escherichia coli ?
-
 ?
4.2.99.18 additional information KsgA does not remove C opposite normal bases, 7,8-dihydro-8-oxoguanine and 2-hydroxyadenine, KsgA does not excise thymine glycol, 5-formyluracil, and 5-hydroxymethyluracil opposite cytosine from double-stranded oligonucleotides Escherichia coli KSR7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.182 ? x * 30400, SDS-PAGE, calculated from sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.182 KsgA
-
 Escherichia coli
4.2.99.18 KsgA a 16S rRNA adenine methyltransferase with DNA glycosylase/AP lyase activity Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.182 physiological function KsgA has a DNA glycosylase/AP lyase activity for C mispaired with oxidized T that prevents the formation of mutations, which is in addition to its rRNA adenine methyltransferase activity essential for ribosome biogenesis Escherichia coli